Functional analysis of <scp>ABCG</scp> and <scp>ABCH</scp> transporters from the red flour beetle, <scp> <i>Tribolium castaneum</i> </scp>

Background ATP-binding cassette transporter (ABC transporter) subfamilies ABCA-C and ABCG-H have been implicated in insecticide detoxification, mostly based on findings of elevated gene expression response to treatment. We previously characterized TcABCA-C genes from the model beetle pest Tribolium castaneum demonstrated that TcABCA TcABCC are involved elimination diflubenzuron, because RNA interference (RNAi)-mediated silencing increased susceptibility. In this study, we focused potential functions TcABCG TcABCH detoxification. Results When silenced TcABCG-H using RNAi, noticed a unreported developmental RNAi phenotype for TcABCG-4F, which is by 50% mortality ecdysial arrest during adult moult. knocked down Drosophila brown orthologue TcABCG-XC, did not obtain apparent eye colour phenotypes but observe loss riboflavin uptake Malpighian tubules. Next, determined profiles all different tissues stages analysed transcript levels treatment with four chemically unrelated insecticides. found some were specifically upregulated after However, when insecticide-induced mortalities larvae treated double-stranded injection silence those upregulated, significant increase susceptibility Conclusion Our suggest observed insecticide-dependent induction reflects an unspecific stress response, hence underlines significance functional studies © 2021 The Authors. Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.